【个人简介】
张元兴:博导 教授 国家863计划领域专家 
    山东省莱州市人。1982年1月毕业于华东化工学院化学工程系，获学士学位。1984年7月毕业于华东化工学院生物化工系，获硕士学位，1995年9月评为华东理工大学教授。1997年评为博士生导师。享受国务院政府特殊津贴。

现任职务有上海阿华生物工程研究所所长，国家863高技术计划海洋生物技术主题专家组副组长，生物反应器工程国家重点实验室学术委员会委员。

【主要教学科研成果】
为硕士研究生讲授动物细胞培养课程，为博士研究生开设生物反应器设计与放大课程，已指导21名硕士研究生和12名博士研究生毕业获得学位，正在指导硕士研究生11名，博士研究生10名，博士后1名。已在国内外发表论文150余篇，出版专著一部，获省部级科技进步奖2项，申请中国发明专利10项，美国专利1项。

【论文发表情况】
2004年以来发表和出版中论文：
1. Xiangming Sun, Yuanxing Zhang* (2004) Glutamine cannot support recombinant CHO cell growth and maintenance in the absence of glucose. Process Biochemistry 39(6):719~722.
2. Li Zhang, Yuanxing Zhang (2004) Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy. Journal of Chemical Technology and Biotechnology 79(2):171~181.
3. Xiaoping Yi, Xiangming Sun, Yuanxing Zhang* (2004) Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression. Process Biochemistry 39(11):1817~1823.
4. Qin Liu, Yue Ma, Haizhen Wu, Mingfei Shao, Haifeng Liu, Yuanxing Zhang* (2004) Cloning, identification and expression of an entE homologue angE from Vibrio anguillarum serotype O1. Archives of Microbiology 181(4):287~293.
5. Weibin Zhou, Yuanxing Zhang* (2004) Purification and identification of recombinant hirudin and its degradation derivatives expressed in Pichia pastoris. Preparative Biochemistry & Biotechnology 34(3):239~252.
6. Jizhong Yang, Xiangshan Zhou, Yuanxing Zhang* (2004) Improvement of recombinant hirudin production by controlling NH4+ concentration in Pichia pastoris fermentation. Biotechnology Letter 26(12):1013~1017.
7. Haizhen Wu, Yue Ma, Yuanxing Zhang, Huizhan Zhang (2004) Complete sequence of virulence plasmid pEIB1 from the marine fish pathogen Vibro anguillarum strain MVM425 and location of its replication region. Journal of Applied Microbiology 97(5):1021~1028
8. Qin Liu, Yue Ma, Lingyun Zhou, Yuanxing Zhang* (2005) Gene cloning and functional characterization of a phosphopantetheinyl transferase from Vibrio anguillarum serotype O1. Achieves of Microbiology 183(1):37~44.
9. Subiao Lu, Xiangming Sun, Yuanxing Zhang* (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochemistry 40(5):1917~1921.
10. Juxing Chen, Xiangming Sun, Yuanxing Zhang* (2005) Growth and metabolism of marine fish Chinook salmon embryo cells: Response to lack of glucose and glutamine. Biotechnology Letters 27(6): 395~401.
11. Juxing Chen, Xiangming Sun, Li Zhang, Xiaoping Yi, Yuanxing Zhang* (2005) Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium. Aquaculture 249(1-4):35~45.
12. Hechun Zhang, Jixun Zhan, Keman Su, Yuanxing Zhang* (2006) A kind of potential food additive produced by Streptomyces coelicolor: characteristics of blue pigment and identification of a novel compound, l-actinorhodin. Food Chemistry 95(2):186~192.
13. Junhui Li, Zhengyu Huang, Xiangming Sun, Pengyuan Yang, Yuanxing Zhang* (2006) Understanding the enhanced effect of dimethyl sulfoxide on hepatitis B surface antigen expression in the culture of Chinese hamster ovary cells on the basis of proteome analysis. Enzyme and Microbiol Technology, in press.
14. Weiwen Luo, Xiangming Sun*, Xiaoping Yi, Yuanxing Zhang (2006) Enhancement of hepatitis B surface antigen production by adenosine 5‘-monophosphate in the culture of recombinant Chinese hamster ovary cells. Journal of Bioscience and Bioengineering, in press.
15. Mingfei Shao, Yue Ma, Qin Liu, Yuanxing Zhang* (2006) Secretory expression of recombinant proteins in an attenuated Vibrio anguillarum strain for potential use in vaccines. Journal of Fish Diseases, in press.
16. Junhui Li, Xiangming Sun, Yuanxing Zhang* (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochemistry, in press.
17. Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang* (2006) A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative. Biotechnology Letters,  accepted.

【研究方向】
(1) 动物细胞培养。通过动物细胞生长周期的调节，结合营养物的富集和平衡以及流加和灌注培养模式的采用，提高动物细胞密度和产物产量。通过研究动物细胞生长、代谢特征和血清在细胞培养中的作用，寻求优化的培养基组成，开发分别适合贴壁和悬浮细胞培养的无血清和无蛋白培养基。研究细胞培养方法和分离纯化条件对药用蛋白质糖基化的影响，从生物制药整体过程上进行优化，提高药用蛋白的分子均一性。目前承担着国家863计划研究任务。
(2) 海洋生物技术。研究海洋鱼类病原微生物的特性，掌握其毒力因子和致病规律，确定致病相关功能基因，从基因水平上进行改造，开发减毒活疫苗。目前承担着国家863计划研究项目。
(3) 生物制药工程。利用生物反应器，通过基因重组微生物的高密度发酵和基因调控，高表达重组产物，进行蛋白质的大规模纯化，生产高纯度的药用蛋白。在甲醇酵母的发酵中，研究基因诱导水平的精密控制和关键酶的合成，产物蛋白的酶水解机制，提高产物蛋白的表达。目前承担着国家863计划研究任务和企业委托项目。

【联系方式】
电话：64253025
Email：yxzhang@ecust.edu.cn